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技術(shù)文章Article 首 頁(yè) > 技術(shù)文章 > 6T-CEM 人T細(xì)胞白血病細(xì)胞
6T-CEM 人T細(xì)胞白血病細(xì)胞
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6T-CEM   人T細(xì)胞白血病細(xì)胞

General Information:

Organism:

Homo sapiens, human

Tissue:

peripheral blood

Culture Properties:

suspension

Morphology:

lymphoblast

Biosafety Level:

2

 

Culture Method:

Complete Growth Medium:

RPMI-1640(Gibco31800-022)+10%FBS+ Penicillin/Streptomycin

Culture Conditions:

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C

Subculturing:

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1×105 viable cells/mL. Do not allow cell concentration to exceed 1×106 cells/mL.

Interval: Maintain cell density between 1×105 and 1×106 viable cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation:

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase